During the structural determination of transmembrane proteins, one crucial piece of information is lost: the orientation of the protein within the lipid bilayer. The TmDet algorithm uniquely leverages atomic coordinates to determine the transmembrane protein's localization within the membrane. Additionally, it finds out the sequential arrangement of transmembrane and re-entrant regions, and identifies interfacial helices parallel to the membrane surface in both the structure and the amino acid sequence. The current version can also predict the membrane localization of proteins embedded in double membranes and identify curved membrane localization. Furthermore, the algorithm is designed to detect potential errors in modeled transmembrane protein structures too.
Utilizing the TmDet algorithm, we update the PDBTM database in conjunction with the PDB's weekly releases. Moreover, this algorithm was employed in the creation of the TmAlphaFold database. For more details about the algorithm, please refer to the Description page. The Usage page provides instructions on how to use the server. Protein structure atomic coordinates can be uploaded and analyzed on the Jobs page. Results are visualized by an integrated Mol* viewer.